A study on creating an attenuated Aeromonas hydrophila mutant strain by knocking out aroA gene
نویسندگان
چکیده
منابع مشابه
Molecular characterization of the Aeromonas hydrophila aroA gene and potential use of an auxotrophic aroA mutant as a live attenuated vaccine.
The aroA gene of Aeromonas hydrophila SO2/2, encoding 5-enolpyruvylshikimate 3-phosphate synthase, was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. The nucleotide sequence of the A. hydrophila aroA gene encoded a protein of 440 amino acids which showed a high degree of homology to other bacterial AroA proteins...
متن کاملBehavior of an Aeromonas hydrophila aroA live vaccine in water microcosms.
Genetically modified auxotrophic mutants of different fish pathogens have been used as live vaccines in laboratory experiments, but the behavior of the strains after release into aquatic ecosystems has not been characterized. We previously constructed and characterized an aroA mutant of Aeromonas hydrophila and studied the protection afforded by this mutant as a live vaccine in rainbow trout. I...
متن کاملAeromonas hydrophila
Department of Biology, University of Alabama a t Birmingham, Birmingham, AL 35294-1 170, USA Aeromonas hydrophila, a gastrointestinal pathogen of humans, was shown to exhibit a significant adaptive acid tolerance response (ATR) capable of protecting cells from severe acid at a pH of 3.5. The ATR was induced by exposure to a relatively mild pH level of 5-0 for 20 min. Adaptation required protein...
متن کاملAeromonas hydrophila lateral flagellar gene transcriptional hierarchy.
Aeromonas hydrophila AH-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. Our results indicate that A. hydrophila lateral flagellar genes are transcribed at three levels (class I to III genes) and share some similarities with, but have many important differences from, genes of Vibrio parahaemolyticus. A. hydrophila lateral...
متن کاملGenome Sequence of Hypervirulent Aeromonas hydrophila Strain HZAUAH
Aeromonas hydrophila, a zoonotic bacterium found in an expansive range of aquatic ecosystems, has been reported to cause severe diseases in fish, amphibians, reptiles, and mammals, including humans. Herein, we report the draft genome of the hypervirulent A. hydrophila strain HZAUAH isolated from a crucian in China.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: TAP CHI SINH HOC
سال: 2014
ISSN: 0866-7160,0866-7160
DOI: 10.15625/0866-7160/v36n1se.4335